Medical science advances at an astonishing pace on all fronts. The area of diagnostics is one particular field where great efforts are made to devise tests and procedures that can help doctors to diagnose diseases quicker and more accurately. This is important because successful treatment often depends upon an early diagnosis. ELISA kits, for example, have empowered doctors to efficiently detect a wide range of diseases.
This revolutionary technique, also known as EIA, or enzyme immunoassay, is conducted in a laboratory. It is used to determine the concentration of antigens or antibodies in a specific sample. The presence and concentration of antibodies is an important indication of the presence of a variety of medical conditions. The test is extremely popular because it is easy to do and many different tests can be processed at the same time.
The ability to identify the presence of either antibodies or antigens in the body is very important. This is because antigens are known to be harmful substances. As soon as the body recognizes the presence of antigens, it starts to produce antibodies to neutralize the harmful antigens. When either of these agents is present in a sample, the existence of a disease can be deduced logically.
There is no need to stress about this type of test. Patients are required to provide a small blood sample. This sample is then added to a petri dish that contains antigens typical to the condition for which the patient is tested. If the blood sample combines with the antigen, antibodies are present and this confirms the diagnosis. The amount of antibodies is determined by carefully adding enzymes to the sample.
The EIA test provides a wealth of information, even when administered in its simplest form. However, more complex techniques can provide even better results. Different formats, or kits, are used for the different forms of the test. For example, in the direct EIA test the antigen is coated with a multiwall plate. The antibody can only detect it if the latter has been conjugated with an enzyme.
Then there is the indirect EIA test. In this case the antigen is covered with a polystyrene plate that can only be detected in two distinct stages. The main advantage of this format of the test is that it offers increased sensitivity. Both primary and secondary antibodies can be identified. This entails cost savings and it may even eliminate the need for further tests.
In the case of yet another type of EIA test, the sandwich test, pairs of antibodies are tested. The first antibody is covered with a polystyrene plate. The next antibody is then added in order to determine the concentration of the analyte. This is a complicated procedure but it is extremely accurate and flexible.
Nobody will deny the fact that diseases are easier and cheaper to treat if they can be diagnosed at an early stage. Tests such as the EIA help doctors to do just this. Conditions such as HIV, pernicious anemia, syphilis, and many others have been diagnosed early enough to save the lives of the patient, all thanks to this amazing procedure.
This revolutionary technique, also known as EIA, or enzyme immunoassay, is conducted in a laboratory. It is used to determine the concentration of antigens or antibodies in a specific sample. The presence and concentration of antibodies is an important indication of the presence of a variety of medical conditions. The test is extremely popular because it is easy to do and many different tests can be processed at the same time.
The ability to identify the presence of either antibodies or antigens in the body is very important. This is because antigens are known to be harmful substances. As soon as the body recognizes the presence of antigens, it starts to produce antibodies to neutralize the harmful antigens. When either of these agents is present in a sample, the existence of a disease can be deduced logically.
There is no need to stress about this type of test. Patients are required to provide a small blood sample. This sample is then added to a petri dish that contains antigens typical to the condition for which the patient is tested. If the blood sample combines with the antigen, antibodies are present and this confirms the diagnosis. The amount of antibodies is determined by carefully adding enzymes to the sample.
The EIA test provides a wealth of information, even when administered in its simplest form. However, more complex techniques can provide even better results. Different formats, or kits, are used for the different forms of the test. For example, in the direct EIA test the antigen is coated with a multiwall plate. The antibody can only detect it if the latter has been conjugated with an enzyme.
Then there is the indirect EIA test. In this case the antigen is covered with a polystyrene plate that can only be detected in two distinct stages. The main advantage of this format of the test is that it offers increased sensitivity. Both primary and secondary antibodies can be identified. This entails cost savings and it may even eliminate the need for further tests.
In the case of yet another type of EIA test, the sandwich test, pairs of antibodies are tested. The first antibody is covered with a polystyrene plate. The next antibody is then added in order to determine the concentration of the analyte. This is a complicated procedure but it is extremely accurate and flexible.
Nobody will deny the fact that diseases are easier and cheaper to treat if they can be diagnosed at an early stage. Tests such as the EIA help doctors to do just this. Conditions such as HIV, pernicious anemia, syphilis, and many others have been diagnosed early enough to save the lives of the patient, all thanks to this amazing procedure.
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